The effect of tacrolimus on reactive oxygen species andtotal antioxidant status in pancreatic beta cell line
Ali M. Sharifi
Mohamad Mehdi Namvaran
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© Başkent University 2015 Printed in Turkey. All Rights Reserved. Objectives: The mechanism responsible for the development of posttransplant diabetes mellitus associated with tacrolimus treatment remains unclear. To investigate the possible effect of tacrolimus on the development of impaired glucose tolerance in transplant recipients, this study focused on early and second phase insulin secretion, which may be affected by reactive oxygen species under tacrolimus therapy. Materials and Methods: We measured the antioxidant status and reactive oxygen species levels before and after tacrolimus treatment. The production of reactive oxygen species was determined by flow cytometry. Total antioxidant status was measured using total antioxidant status kits. Results: The antioxidant status decreased while reactive oxygen species level increased significantly (P = .032). Conclusions: Tacrolimus treatment resulted in doseand time-dependent increases in the production of reactive oxygen species by beta cells. The anti - oxidant status decreased in beta cells after tacrolimus treatment. Increased production of reactive oxygen species and decreased antioxidant status by tacrolimus in beta cells may lead to some adverse events.