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dc.contributor.authorAdel Spotin
dc.contributor.authorAdel Spotin
dc.contributor.authorAdel Spotin
dc.contributor.authorSoheila Rouhani
dc.contributor.authorParnazsadat Ghaemmaghami
dc.contributor.authorParnazsadat Ghaemmaghami
dc.contributor.authorAli Haghighi
dc.contributor.authorMohammad Reza Zolfaghari
dc.contributor.authorAref Amirkhani
dc.contributor.authorMahin Farahmand
dc.contributor.authorAli Bordbar
dc.contributor.authorParviz Parvizi
dc.date.accessioned2017-09-18T10:48:19Z
dc.date.available2017-09-18T10:48:19Z
dc.date.issued2015-07-01
dc.identifier.urihttp://dsp.sbmu.ac.ir/xmlui/handle/123456789/67009
dc.description.abstract© 2015, Pasteur Institute of Iran. All rights reserved. Background: Molecular diversity of Leishmania major and its morphological changes have become a controversial issue among researchers. Some aspects of polymorphic shapes of amastigotes in clinical manifestations along with molecular variation were evaluated among suspected patients of some exceptional zoonotic cutaneous leishmaniasis locations in Northern Khuzestan, Southwestern Iran. Methods: Suspected patients (n = 165) were sampled in zoonotic cutaneous leishmaniasis foci over two consecutive years during 2012- 2014. Prepared smears were stained, scaled and measured by ocular micrometer. DNA was extracted from smears; ITS-rDNA and Cytochrome b (Cyt b) markers were amplified, and PCR products were digested by BsuR1 restriction enzyme. Then the RFLP and sequencing were employed. Results: Only L. major was identified in patients containing regular amastigotes' shapes (oval or round) with a size of 2-4 μm in each of classical wet, dry, mixed lesions. Meanwhile, irregular shapes (spindle, pear, or cigarette) were observed separately in non-classical wet lesions with more than 4 μm. Interestingly, a few amastigotes with an external flagellum were observed in some lesions. All sequenced ITS-rDNA and Cyt b genes of L. major did not show any molecular variation (χ 2 P > 0.05), including only one common haplotype (GenBank access no. EF413075). Conclusion: Findings proved that unlike other endemic foci, there is not a meaningful correlation between phenotypic and genotypic features of L. major isolates. This study is considered as the first comprehensive report to incriminate morphometric shapes of L. major amastigotes, which enhances our knowledge concerning their relevance with various clinical appearances and genotypic traits.
dc.sourceIranian Biomedical Journal
dc.subjectAmastigote shapes
dc.subjectIran
dc.subjectLeishmania major
dc.subjectMitochondrial gene
dc.subjectNuclear gene
dc.titleDifferent morphologies of leishmania major amastigotes with no molecular diversity in a neglected endemic area of zoonotic cutaneous leishmaniasis in Iran
dc.journal.volume19
dc.journal.issue3
dc.journal.pages149-159
dc.contributor.authorid54416426700
dc.contributor.authorid54416426700
dc.contributor.authorid54416426700
dc.contributor.authorid54407388200
dc.contributor.authorid56707298300
dc.contributor.authorid56707298300
dc.contributor.authorid6603854419
dc.contributor.authorid56541204300
dc.contributor.authorid16303318400
dc.contributor.authorid8504428500
dc.contributor.authorid55759227700
dc.contributor.authorid22136163700
dc.contributor.citation54416426700|60005548|Adel Spotin
dc.contributor.citation54416426700|60005548|Adel Spotin
dc.contributor.citation54416426700|60005548|Adel Spotin
dc.contributor.citation54407388200|60018934|Soheila Rouhani
dc.contributor.citation56707298300|60005548|Parnazsadat Ghaemmaghami
dc.contributor.citation56707298300|60005548|Parnazsadat Ghaemmaghami
dc.contributor.citation6603854419|60018934|Ali Haghighi
dc.contributor.citation56541204300|60031777|Mohammad Reza Zolfaghari
dc.contributor.citation16303318400|60102242|Aref Amirkhani
dc.contributor.citation8504428500|60005548|Mahin Farahmand
dc.contributor.citation55759227700|60005548|Ali Bordbar
dc.contributor.citation22136163700|60005548|Parviz Parvizi
dc.contributor.affiliationid60005548
dc.contributor.affiliationid60005548
dc.contributor.affiliationid60005548
dc.contributor.affiliationid60018934
dc.contributor.affiliationid60005548
dc.contributor.affiliationid60005548
dc.contributor.affiliationid60018934
dc.contributor.affiliationid60031777
dc.contributor.affiliationid60102242
dc.contributor.affiliationid60005548
dc.contributor.affiliationid60005548
dc.contributor.affiliationid60005548


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