Relationship between salivary and plasma level of homocysteine in coronary artery disease
Hamid Reza Khalighi
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© by Wroclaw Medical University and Polish Dental Society. Background. It is believed that an elevated plasma homocysteine concentration conferred an independent risk of cardiovascular disease and plasma homocystine level can predict the mortality rate of patients suffering from cardiac disease. Objectives. We designed this study to evaluate the relationship between salivary and plasma level of homocysteine in cardiovascular patients and control group. Material and Methods. We obtained 5 mL blood and 2.5 mL saliva from 34 patients with coronary artery diseases (CAD) and 32 healthy individuals and detected homocysteine in their blood and saliva by high pressure liquid chromatography (HPLC) method. The difference between homocysteine level of patient and control group was compared with t-test. In addition, Pearson-coefficient was also used to determine the relationship between salivary and plasma level of homocysteine. Results. The average of plasma homocysteine level was 15.43 ± 5.07 mmol/Lit in patients and 9.95 ± 5.88 mmol//Lit in the control group; their difference was statistically significant when compared with the use of Student t-test (p < 0.001). The average of salivary homocysteine level was 0.24 ± 0.056 mmol/Lit in patients and 0.023 ± 0.013 mmol//Lit in the control group; their difference was statistically significant (p < 0.001) with the use of Student t-test. There was a relationship between saliva and plasma level of homocysteine in both groups (Person-coefficient = 0.744) Conclusions. There was a relationship between saliva and plasma level of homocysteine, so it can be used as an alternative media for detecting and measuring homocysteine.