Comparison of adherence patterns of a selection of probiotic bacteria to Caco-2, HEp-2, and T84 cell lines
Sayyed Mohammad Hossein Ghaderian
Akram Sadat Tabatabaei Panah
Reza Akbarzadeh Najar
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Adherence is a primary prerequisite for bacterial colonisation and has been considered as one of the main criteria for selection of probiotic bacteria. Different cell lines have been used to investigate probiotic-host interactions in vitro. The comparison of adherence properties of probiotics among different cell lines has not yet been reported. The purpose of this study was to investigate and compare the adherence level and adherence pattern of a selection of probiotic bacteria to Caco-2, HEp-2 and T84 cell lines. The methods used compared probiotic bacteria adherence level and pattern among the cell lines. Eleven lactobacilli or bifidobacteria, isolated from pharmaceutical or dairy probiotic products, and five type strains were tested. Strains of Escherichia coli with known adherence patterns were used as controls. Adhesion assays were carried out using light microscopy. According to the number of cells in each field, and the number of bacteria attached to each of those cells, the adherence level of each strain was classified and compared among cell lines. Also, it was noted that probiotic bacteria showed a localised or diffused pattern of adherence, resembling that seen with locally or diffusely adherent E. coli. Patterns were strain not species dependent. The adherence pattern and level of 14 out of 16 of the strains were similar on HEp-2 and Caco-2. The similarity observed between T84 and Caco-2 was 10 out of 16. This study suggests the potential of using easily handled, rapidly growing HEp-2 cells for primary selection of epithelial adherence of probiotic bacteria. © Springer-Verlag and the University of Milan 2011.