Sequence diversity in tRNA gene locus A-L among Iranian isolates of entamoeba dispar

Date
2012-05-10Author
E. Nazemalhosseini-Mojarad
M. Azimirad
Z. Nochi
S. Romani
M. Tajbakhsh
M. Rostami-Nejad
A. Haghighi
M. R. Zali
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Background: A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates. Methods: A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced.The sequences obtained were edited manually and aligned using Gene Runner software. Results: With sequencing of PCR products a reliable genetic diversity in size, number and position of the repeat units were observed among the Iranian E. dispar isolates in locus A-L gene. Sequences showed variation in length from 448bp to 507bp and seven distinct types were identified. Conclusion: The genetic diversity of loci like A-L shows them to be suitable for pidemiological studies such as the characterization of the routes of transmission of these parasites in Iran.