Advantage of applying OSC to 1H NMR-based metabonomic data of celiac disease
Mohamad Reza Zali
Mohamad Rostami Nejad
Afsaneh Arefi oskouie
Seyed Abdol Reza Mortazavi-Tabatabaei
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Background: Celiac disease (CD) is a disorder associated with body reaction to gluten. After the gluten intake, an immune reaction against the protein occurs and damages villi of small intestine in celiac patients gradually. Objectives: The OSC, a filtering method for minimization of inter- and intra-spectrom-eter variations that influence on data acquisition, was applied to biofluid NMR data of CD patients. Patients and Methods: In this study, metabolites of total 56 serum samples from 12 CD patients, 15 CD patients taking gluten-free diet (GFD), and 29 healthy cases were analyzed using nuclear magnetic resonance (NMR) and associated theoretical analysis. Employ-ing ProMetab (version ProMetab_v3_3) software, data obtained from NMR spectra were reduced and orthogonal signal correction (OSC) effect on celiac disease metabonomics before and after the separation by principle component analysis (PCA) was investigated. Results: The three groups were separated by OSC and findings were analyzed by partial least squares discriminant analysis (PLS-DA) method. Root mean square error of calibra-tion (RMSEc) and correlation coefficient of calibration (Rc) for PLS-DA referred to an ef-ficient group separation filtered by OSC. Conclusions: The applied leave-one-out cross-validation to PLS-DA method performed along with OSC confirmed validation of data analysis. Finally four metabolites are intro-duced as CD biomarkers. © 2012 RIES & IES. Publish by Kowsar Corp.