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dc.contributor.authorFatemeh Sanie-Jahromi
dc.contributor.authorHamid Ahmadieh
dc.contributor.authorZahra Soheila Soheili
dc.contributor.authorMaliheh Davari
dc.contributor.authorShima Ghaderi
dc.contributor.authorMozhgan Rezaei Kanavi
dc.contributor.authorShahram Samiei
dc.contributor.authorAbdolkhalegh Deezagi
dc.contributor.authorJalil Pakravesh
dc.contributor.authorAbouzar Bagheri
dc.date.accessioned2017-09-18T10:00:13Z
dc.date.available2017-09-18T10:00:13Z
dc.date.issued2012-06-19
dc.identifier.issn17560500
dc.identifier.urihttp://dsp.sbmu.ac.ir/xmlui/handle/123456789/61168
dc.description.abstractBackground: Retinal progenitor cells are a convenient source of cell replacement therapy in retinal degenerative disorders. The purpose of this study was to evaluate the expression patterns of the homeobox genes PAX6 and CHX10 (retinal progenitor markers) during treatment of human retinal pigment epithelium (RPE) cells with amniotic fluid (AF), RPE cells harvested from neonatal cadaver globes were cultured in a mixture of DMEM and Ham's F12 supplemented with 10% FBS. At different passages, cells were trypsinized and co-cultured with 30% AF obtained from normal fetuses of 1416 weeks gestational age. Results: Compared to FBS-treated controls, AF-treated cultures exhibited special morphological changes in culture, including appearance of spheroid colonies, improved initial cell adhesion and ordered cell alignment. Cell proliferation assays indicated a remarkable increase in the proliferation rate of RPE cells cultivated in 30% AFsupplemented medium, compared with those grown in the absence of AF. Immunocytochemical analyses exhibited nuclear localization of retinal progenitor markers at a ratio of 33% and 27% for CHX10 and PAX6, respectively. This indicated a 3-fold increase in retinal progenitor markers in AF-treated cultures compared to FBS-treated controls. Real-time PCR data of retinal progenitor genes (PAX6, CHX10 and VSX-1) confirmed these results and demonstrated AF's capacity for promoting retinal progenitor cell generation. Conclusion: Taken together, the results suggest that AF significantly promotes the rate of retinal progenitor cell generation, indicating that AF can be used as an enriched supplement for serum-free media used for the in vitro propagation of human progenitor cells. © 2012 Sanie-Jahromi et al; licensee BioMed Central Ltd.
dc.sourceBMC Research Notes
dc.subjectAge related macular degeneration (AMD)
dc.subjectAmniotic fluid
dc.subjectCellular therapy
dc.subjectRetinal progenitor cells
dc.subjectSerum-free
dc.titleEnhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid
dc.journal.volume5
dc.identifier.doi10.1186/1756-0500-5-182
dc.journal.pages
dc.contributor.authorid55249729500
dc.contributor.authorid6701702031
dc.contributor.authorid56593248000
dc.contributor.authorid50960904100
dc.contributor.authorid50961289800
dc.contributor.authorid14012651100
dc.contributor.authorid57194085284
dc.contributor.authorid6504041890
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dc.contributor.authorid55251138800
dc.contributor.citation55249729500|60019580|Fatemeh Sanie-Jahromi
dc.contributor.citation6701702031|60018934|Hamid Ahmadieh
dc.contributor.citation56593248000|60019580|Zahra Soheila Soheili
dc.contributor.citation50960904100|60019580|Maliheh Davari
dc.contributor.citation50961289800|60019580|Shima Ghaderi
dc.contributor.citation14012651100|60018934|Mozhgan Rezaei Kanavi
dc.contributor.citation57194085284|60089241|Shahram Samiei
dc.contributor.citation6504041890|60019580|Abdolkhalegh Deezagi
dc.contributor.citation6504782289|101671692|Jalil Pakravesh
dc.contributor.citation55251138800|60019580|Abouzar Bagheri
dc.contributor.affiliationid60019580
dc.contributor.affiliationid60018934
dc.contributor.affiliationid60019580
dc.contributor.affiliationid60019580
dc.contributor.affiliationid60019580
dc.contributor.affiliationid60018934
dc.contributor.affiliationid60089241
dc.contributor.affiliationid60019580
dc.contributor.affiliationid101671692
dc.contributor.affiliationid60019580


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