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dc.contributor.authorF. Kheirandish
dc.contributor.authorM. Bandehpour
dc.contributor.authorM. Bandehpour
dc.contributor.authorA. Haghighi
dc.contributor.authorF. Mahboudi
dc.contributor.authorM. Mohebali
dc.contributor.authorB. Kazemi
dc.contributor.authorB. Kazemi
dc.date.accessioned2017-09-18T10:00:13Z
dc.date.available2017-09-18T10:00:13Z
dc.date.issued2012-06-20
dc.identifier.urihttp://dsp.sbmu.ac.ir/xmlui/handle/123456789/61167
dc.description.abstractBackground: Protozoa related to Trypanosome family including Leishmania, synthesize enzymes to escape from drug therapy. One of them is PTR1 that its enzymatic activity is similar to dihydrofolate reductase (DHFR).Dihydrofolate reductase - thymidylate synthase has a major role in DNA synthesis, if it is inhibited, the result would be the death of parasite. Since PTR1 activity is similar to DHFR, causes the decrease of inhibition effect of drug. The aim of this study was inhibition of Iranian L. major PTR1 expression with mRNA antisense in prokaryotic system as an approach to appear of the drugs therapeutic effects more. Methods: PTR1 gene was ligated to pACYCDuet-1 and pcDNA3 plasmids as sense and antisense plasmids, respectively. Simultaneously transfer of sense and antisense plasmids was done in E. coli strain M15. SDS-PAGE and western blot analysis were carried out to analyze the expression. Results: Sense and antisense plasmids were prepared and confirmed by restriction analysis and PCR then simultaneously transfer of them was done. SDS-PAGE and western blot analysis showed PTR1 gene was inhibited by mRNA antisense in bacterial cells. Conclusion: Expression of PTR1 gene in sense plasmid was inhibited successfully by antisense plasmid.
dc.sourceIranian Journal of Public Health
dc.subjectAntisense plasmid
dc.subjectDihydrofolate reductase
dc.subjectExpression
dc.subjectLeishmania major
dc.subjectPTR1
dc.titleInhibition of leishmania major PTR1 gene expression by antisense in Escherichia coli
dc.journal.volume41
dc.journal.issue6
dc.journal.pages65-71
dc.contributor.authorid34768244600
dc.contributor.authorid16641881900
dc.contributor.authorid16641881900
dc.contributor.authorid6603854419
dc.contributor.authorid6603242520
dc.contributor.authorid6602905867
dc.contributor.authorid6602441752
dc.contributor.authorid6602441752
dc.contributor.citation34768244600|60015746|F. Kheirandish
dc.contributor.citation16641881900|60018934|M. Bandehpour
dc.contributor.citation16641881900|60018934|M. Bandehpour
dc.contributor.citation6603854419|60018934|A. Haghighi
dc.contributor.citation6603242520|60005548|F. Mahboudi
dc.contributor.citation6602905867|60027708|M. Mohebali
dc.contributor.citation6602441752|60018934|B. Kazemi
dc.contributor.citation6602441752|60018934|B. Kazemi
dc.contributor.affiliationid60015746
dc.contributor.affiliationid60018934
dc.contributor.affiliationid60018934
dc.contributor.affiliationid60018934
dc.contributor.affiliationid60005548
dc.contributor.affiliationid60027708
dc.contributor.affiliationid60018934
dc.contributor.affiliationid60018934


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