Beneficial effect of a sturgeon-based bioactive compound on gene expression of tumor necrosis factor-α, matrix metalloproteinases and type-10 collagen in human chondrocytes
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In the present study, we examined the effect of a marine bioactive compound containing high-purity caviar-derived DNA, collagen elastin and protein extracts from sturgeon (LD-1227, Caviarlieri, Laboratoires Dom, Switzerland) on ILβ-induced activation and production of TNFα and MMP-13 in human osteo-arthritis (OA) chondrocytes and intracellular signaling factors. Human chondrocytes were derived from OA cartilage and stimulated with ILβ. Gene expression of TNFα, MMP-13, MMP-1 and Co110Al was measured by quantitative RT-PCR. TNFα protein in culture medium was determined using cytokine-specific ELISA. Western immunoblotting was used to analyze the MMP-13 production in the culture medium and the activation of NF-κB. DNA binding activity of NF-κB p65 was determined using a highly sensitive and specific ELISA. MMP-13 activity in the culture medium was assayed by gelatine zymography. LD-1227 significantly decreased ILβ-stimulated gene expression and production of TNFα, MMP-1, MMP-13 and Co110Al in human chondrocytes. The inhibitory effect of LD-1227 on the ILβ-induced expression of these genes was mediated at least in part via suppression of NF-κB p65. These data show that LD-1227 can inhibit IL-lβ-induced proliferation and inflammatory reactions via inhibited activation of the transcription factor NF-κB pathway in human chondrocytes derived from OA patients. These novel pharmacological actions of LD-1227 on ILβ-stimulated human OA chondrocytes provide suggestions that this marine biology compound may inhibit cartilage degradation by suppressing ILβ-mediated activation and the catabolic response in human chondrocytes. Copyright © by BIOLIFE, s.a.s.