Direct electron transfer of cytochrome c on ZrO <inf>2</inf> nanoparticles modified glassy carbon electrode
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In this study we used of nanotechnology, electrochemistry and biology for investigation of direct electron transfer of cytochrome c (cyt c) on ZrO 2 nanoparticles (Nps) modified glassy carbon electrode (GCE). Properties of Synthesized ZrO 2 nanoparticles were investigated by X-ray diffraction (XRD) and UV-visible devices. Direct electrochemistry of the modified GCE with Cyt c and ZrO 2 nanoparticles achieved and formal potential (E0) calculated as: -117.5 mV versus reference electrode. Protein structure of cytochrome c with heme center could change oxidative iron state (Fe 3+ ) to reductive state (Fe 2+ ) and vice versa by electron transfer. We used of this electron transfer in designing a biosensor for hydrogen peroxide (H 2 O 2 ) detection. The linear dependence of cathodic peak on the H 2 O 2 concentration was observed in the range of 40 to 270μM; also designed biosensor showed a good reproducibility and stability.© 2012 by ESG.