Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: A possible preventive role for antioxidants
T. R. Neyestani
M. R. Eshraghian
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Background/Objectives:Oxidative stress (OS) is thought to be involved in both development of type 1 diabetes (T1D) and its further complications. In this study, certain biomarkers of OS were compared among the subjects with T1D, their non-diabetic siblings and unrelated healthy controls.Subjects/Methods: Known cases of T1D from both sexes aged 5-25 years were enrolled in a case-control study (n 1 60). There were two control groups; non-diabetic siblings (n 2 60) and unrelated apparently healthy subjects (n 3 60). Anthropometric, dietary and laboratory assessments were done.Results:There was no significant difference in dietary data among the groups. Total antioxidant capacity was significantly lower in T1D than both related and unrelated controls (1.60.05, 1.70.05 and 1.80.06 mmol BSA equivalent/l, respectively, P0.044). Both T1D subjects and their siblings showed lower glutathione peroxidase (GSH-px) levels (median (interquartile range): 22.2 (28.6), 29.9 (23) and 41.8 (73.6) U/ml, respectively, P0.006). On the contrary, superoxide dismutase concentrations were significantly higher in T1D group and the siblings than unrelated healthy controls (243 (45.3), 157.8 (176.9) and 27.9 (8.7) U/l, respectively, P0.001). Serum concentrations of GSH correlated with energy intake in the siblings (r0.521, P0.001) and unrelated controls (r0.268, P0.042) but not in T1D group. The associations remained significant after controlling for blood glucose (r0.437, P0.001 and r0.420, P0.011, respectively) in both the groups.Conclusion:Augmented OS in the siblings may indicate an increased requirement for antioxidants in genetically diabetes-prone subjects. © 2012 Macmillan Publishers Limited All rights reserved.