Mechanism of aspartame-induced antinociception in mice
MetadataShow full item record
Objective: To investigate the possible mechanism of action of aspartame ingestion on antinociception in mouse formalin test. Methods: By applying twelve days regimen of the non-caloric sweetener, aspartame (0.16% w/v) in drinking water, alone or in combination with 1-aminocyclobutane-cis-1,3-dicarboxylic acid (ACBD; NMDA agonist) and 3-((R)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP, NMDA antagonist) at doses of 0.025, 0.05 and 0.1 mg/kg the formalin-induced pain behavior was investigated in mice. Results: ACBD induced hypernociception in the acute phase (1 mg/kg) and late phase (0.5 and 1 mg/kg). CPP exhibited antinociception in acute phase (0.1 mg/kg) and late phase (0.05 and 0.1 mg/kg). Aspartame induced antinociception in both phases of formalin test. When subthreshold doses of ACBD (0.1 mg/kg) and CPP (0.025 mg/kg) were administered to aspartame-treated mice, CPP potentiated and ACBD abolished the aspartame-induced antinociception in both phases of the formalin test respectively. Conclusion: Results suggest that aspartame may induce antinociception through NMDA system.