Rapid determination of metformin in human plasma using ion-pair HPLC
S. M. Foroutan
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A rapid, simple and sensitive ion-pair HPLC method has been developed for quantification of metformin in plasma. The assay enables the measurement of metformin for therapeutic drug monitoring with a minimum detectable limit of 20 ng/ml. The method involves simple, one-step extraction procedure and analytical recovery was complete. The separation was performed on an analytical 150×4.6 mm i.d. μbondapak C 18 column. The wavelength was set at 235 nm. The mobile phase was 40% acetonitrile, 0.01 M sodium dodecyl sulphate, 0.01 M sodium dihydrogen phosphate, and distilled water to 100%, adjusted to pH 5.1 at a flow rate of 1.5 ml/min. The calibration curve was linear over the concentration range 0.2-2.5 μg/ml. The coefficients of variation for inter-day and intra-day assay were within the range of clinical usefulness. © 2002 Elsevier Science B.V. All rights reserved.